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EFFECT OF 5-FLUOROURACIL IN IMPLANTS INSTALLED IN THE RAT TiBIA
EAO Library. Theodoro L. 09/29/16; 164549; PBR246
Leticia Theodoro
Leticia Theodoro
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Abstract
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Background
Oral rehabilitation of cancer patients with dental implants has a positive effect on quality of life after reconstructive surgery. The effect of chemotherapy on implant survival has been discussed in t previous studies. Additional studies of the effect of chemotherapy with 5-Fluorouracil (5-FU) in implants is necessary.

Aim/Hypothesis
The purpose of this study was to evaluate by biomechanical, histomorphometric, immunohistochemical for the detection of tartrate-resistant acid phosphatase immunomarkers (TRAP) and osteocalcin (OCN), and micro-computed tomography analysis the effect of 5-FU in implants installed in the rat tibia.

Material and Methods
Twenty animals were subjected to a surgical procedure for installing a titanium implant in their right tibia and a titanium implant in their left tibia. The animals were randomly assigned into the following experimental groups: Group C30/45D (control; n = 10) - animals received their first injection with saline solution 30 days after implant placement and were euthanized 45 days after implant placement; Group 5FU30/45D (n = 10) - animals received the first dose of 5-FU (60 mg/kg e 40 mg/kg) 30 days after implant placement and were euthanized 45 days after implant placement. The right and left tibia were collected and fixed in buffered formalin 4% and 70% ethanol, respectively. The pieces obtained were processed to perform biomechanical, micro-computed tomography, histomorphometric and immunohistochemical (TRAP and OCN) analyses.

Results
In biomechanical, micro-computed and histometric analyses there were no differences among groups. (p>0.05). There was discreet immunolabeling to TRAP- positive cells in all experimental groups. The immunolabeling pattern to OCN was moderated in all experimental groups and periods.

Conclusions and clinical implications
It was concluded that when chemotherapy with 5-FU is performed after osseointegration of titanium implants it does not exert negative influence on peri-implant tissues in rats.

Background
Oral rehabilitation of cancer patients with dental implants has a positive effect on quality of life after reconstructive surgery. The effect of chemotherapy on implant survival has been discussed in t previous studies. Additional studies of the effect of chemotherapy with 5-Fluorouracil (5-FU) in implants is necessary.

Aim/Hypothesis
The purpose of this study was to evaluate by biomechanical, histomorphometric, immunohistochemical for the detection of tartrate-resistant acid phosphatase immunomarkers (TRAP) and osteocalcin (OCN), and micro-computed tomography analysis the effect of 5-FU in implants installed in the rat tibia.

Material and Methods
Twenty animals were subjected to a surgical procedure for installing a titanium implant in their right tibia and a titanium implant in their left tibia. The animals were randomly assigned into the following experimental groups: Group C30/45D (control; n = 10) - animals received their first injection with saline solution 30 days after implant placement and were euthanized 45 days after implant placement; Group 5FU30/45D (n = 10) - animals received the first dose of 5-FU (60 mg/kg e 40 mg/kg) 30 days after implant placement and were euthanized 45 days after implant placement. The right and left tibia were collected and fixed in buffered formalin 4% and 70% ethanol, respectively. The pieces obtained were processed to perform biomechanical, micro-computed tomography, histomorphometric and immunohistochemical (TRAP and OCN) analyses.

Results
In biomechanical, micro-computed and histometric analyses there were no differences among groups. (p>0.05). There was discreet immunolabeling to TRAP- positive cells in all experimental groups. The immunolabeling pattern to OCN was moderated in all experimental groups and periods.

Conclusions and clinical implications
It was concluded that when chemotherapy with 5-FU is performed after osseointegration of titanium implants it does not exert negative influence on peri-implant tissues in rats.

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